PhD Theses (WII)

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    Genetic assessment of the Non-descript pig breeds across selected regions of Uttarakhand
    (Wildlife Institute of India, Dehradun, 2023) Sahoo, Khushboo; Gupta, S.K.; Gopi, G.V.
    India, which has ancient history of pig rearing, is home to several indigenous pig breeds that reflect the adaptation of Sus scrofa to different environmental and cultural conditions. However, only 13 of these breeds are currently recognized and documented by the National Bureau of Animal Genetic Resources. The present research was undertaken to identify the spatial distribution, socio-economic status, pig husbandry methods of pig rearers, and the impact of Exotic pig breed on the indigenous breed and ecology of the region. As molecular characterization is a valuable tool that provides insights into the evolutionary history and genetic diversity of the current pig breed raise in a region. Hence by incorporating molecular techniques in understanding of the domestic pig breed and their interactions with ecosystems, promote more precise and science-based management that contributes to biodiversity conservation and sustainable husbandry practices. The present study will also aim to evaluate the maternal lineage, genetic diversity and population structure of the domestic pig breeds at the regional scale, using microsatellite markers and mitochondrial DNA sequences. The survey and sampling was done in the planar (Dehradun, Haridwar and Nainital) districts of Uttarakhand, a mountainous state of India that lies in the foothills of Himalayas, where rapid urbanisation and continuous migration make us reconsider the necessity of an organise promotion of the piggery sector due to its multiple ecological implications.
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    Genetic diversity, gene flow and mating system in sympatric crocodylian species in National Chambal Sanctuary, India
    (Wildlife Institute of India, Dehradun, 2023) Sharma, Surya Prasad
    The present research was, thus, undertaken to provide information on the genetic status of two freshwater crocodylian species through assessment of the (a) genetic diversity, (b) population genetic structure, genetic differentiation, and gene-flow and (c) mating system using nuclear microsatellite and mitochondrial markers. The biological samples for genetic assessment were collected from two of the largest breeding gharial population in Chambal River (within the National Chambal Sanctuary) and Girwa River (within) Katerniaghat Wildlife Sanctuary) for both the study species.
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    Phylogeography and population genetics of greater one-horned rhinoceros (Rhinoceros unicornis) in India
    (wildlife Institute of India, Dehradun, 2022) Ghosh, Tista
    This work aimed to generate an exhaustive genetic database at landscape level for the Indian GoH rhinoceros which can be used for population management plans (like translocation programmes) and scientific evidence in court prosecutions of alleged rhino poaching crimes. The study focuses on assessing the evolutionary history and genetic health of each rhino bearing areas in India to suggest conservation mitigation plans at the park level. Further, it also attempts to generate an allele frequency map of forensic standards for a large trade-targeted mammal. This thesis re-emphasizes the application of genetic data for making long term conservation plans and promotes its inclusion in future efforts as proven in many other cases. The study focuses on securing the genetic diversity of extant populations of Indian rhino by providing information for the targeted conservation efforts. This study was conducted across all the seven rhino bearing area of India distributed in three states of Assam (Kaziranga National Park (NP), Orang NP, Pobitora Wildlife Sanctuary (WLS) and Mans NP), West Bengal (Gorumara NP and Jaldapara NP) and Uttar Pradesh (Dudhwa NP)
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    Meta-population Dynamics of Tiger (Panthera Tigris Tigris) in the Terai-Arc Landscape, India
    (Wildlife Institute of India, Dehradun, 2021) Biswas, Suvankar; Mondol, Samrat
    This thesis focused to add deeper understanding of populations at a large, contiguous landscape Studying the tiger source-sink population dynamics in relation to current habitat scenarios would be crucial to set the site-specific future management strategies for successful tiger conservation mitigation. In this thesis, a combination of intensive field surveys, non-invasive genetic tools and GIS modelling were used to assess the tiger population connectivity across the TAL. More specifically, genetic diversity, population structure, demographic changes, source and sink populations and the habitat features which help to retain such population dynamics were investigated. Objectives: This thesis has three objecives which have formed three separate chapters. i. Population structure and demographic history of tigers in the Terai-Arc landscape, India. ii. Source-sink dynamics of tigers across different protected and nonprotected habitats in the Terai-Arc landscape, India. iii. Assessment of landscape connectivity of tigers in the Terai-Arc landscape, India.
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    Assessment of genetic variation in Sambar deer (Rusa unicolor)
    (wildlife Institute of India, 2014) Gupta, Sandeep Kumar; Hussain, S.A.
    This study is focused on the examination of intra and inter-species genetic variation among Indian sambar (R. u. unicolor) populations using mitochondrial DNA (mtDNA) and nuclear DNA markers. Sequence variation in a partial fragment of mtDNA control region was examined from the biological samples collected from Western Ghats, Deccan Peninsula, Semi-arid and Gangetic plains. It is also essential to know the phylogenetic variations (if any) among sambar population in India for identification of population(s) for proper conservation management. Therefore, the following objectives were set forth: (a) to examine the intra-species relationship among sambar populations across various biogeographic zones in India, (b) to study the molecular systematic of sambar in order to understand its phylogenetic structure and its relationships with other cervids; and (c) to examine the genetic diversity and gene flow pattern among sambar populations. The key research questions were (a) is there any variation in phylogenic position of sambar across biogeographic zones of India? (b) what is the precise phylogenic position of sambar with respect to other cervids? (c) does the population genetic structure of sambar differ across biogeographic zones of India? and (d) what is the status of gene flow in sambar across biogeographic zones? In this study, one ancient evolutionary significant unit (ESU) of sambar was identified from Western Ghats.