PhD Theses (WII)

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Subject: search.filters.subject.Genetic variation

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    Assessment of genetic variation in Sambar deer (Rusa unicolor)
    (wildlife Institute of India, 2014) Gupta, Sandeep Kumar; Hussain, S.A.
    This study is focused on the examination of intra and inter-species genetic variation among Indian sambar (R. u. unicolor) populations using mitochondrial DNA (mtDNA) and nuclear DNA markers. Sequence variation in a partial fragment of mtDNA control region was examined from the biological samples collected from Western Ghats, Deccan Peninsula, Semi-arid and Gangetic plains. It is also essential to know the phylogenetic variations (if any) among sambar population in India for identification of population(s) for proper conservation management. Therefore, the following objectives were set forth: (a) to examine the intra-species relationship among sambar populations across various biogeographic zones in India, (b) to study the molecular systematic of sambar in order to understand its phylogenetic structure and its relationships with other cervids; and (c) to examine the genetic diversity and gene flow pattern among sambar populations. The key research questions were (a) is there any variation in phylogenic position of sambar across biogeographic zones of India? (b) what is the precise phylogenic position of sambar with respect to other cervids? (c) does the population genetic structure of sambar differ across biogeographic zones of India? and (d) what is the status of gene flow in sambar across biogeographic zones? In this study, one ancient evolutionary significant unit (ESU) of sambar was identified from Western Ghats.
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    Demographic status and genetic variation of sangai in in Keibul Lamjao National Park, Manipur.
    (Wildlife Institute of India, Dehradun, 2012) Angom, Sangeeta; Hussain, S.A.
    The present study was intended to improve upon the existing ecological and genetic knowledge base of the severely fragmented and isolated population of sangai in the Keibul Lamjao National Park. The major objectives of the study were to estimate the abundance and demographic parameters of sangai and the associated cervids in the Park and to assess the genetic variability using mtDNA (cytochrome b and control region) genes and polymorphic microsatellite markers so as to develop appropriate measures for the conservation of this endangered species. The demographic status of sangai and hog deer in Keibul Lamjao National Park was determined by conducting the population estimation exercise during 2006 - 2008 using point count method. Deers were counted from eighteen bamboo machans and four observation points.