Unveiling Torputitora presence in Sikkim streams through eDNA

Abstract

Environmental DNA (eDNA) metabarcoding was used to detect the presence of Tor putitora (Golden mahseer) in selected streams of Sikkim. Water samples were collected from six sites based on habitat characteristics conducive to the species survival. The collection of samples was followed by filtration of water samples, isolation of the DNA, PCR and sequencing targeting the COI region were conducted under aseptic conditions. The results confirmed Tor putitora presence at two locations (BR-06 and BR-08) with high certainty (>99%) aligning with previous studies on tis distribution in Sikkim. These results indicate that these streams serve as habitats for Tor putitora. These findings highlight the potential of these streams to support mahseer populations, reinforcing the need for conservation efforts to protect these critical habitats from ecological threats like ecological pressures, including hydropower and linear infrastructure development. This study adds to the expending evidence concerning mahseer distribution in Sikkim and highlights the critical need for conservation initiatives aimed at safeguarding its essential habitats by incorporating appropriate mitigation measures. Continued research and long term monitoring are imperative to evaluate population dynamics and ensure the sustainable management of Tor putitora in the region.